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Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP

We thus hypothesize that myocardial mitochondrial membrane cholesterol enrichment due to ischemia may be one of the mechanisms of mGSH depletion in ischemic myocardium

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This method delivers glutathione directly into the bloodstream, ensuring maximum absorption and efficacy